The dominance degree of tocopherol contents in F1 sunflower hybrids

Abstract

The aim and tasks of the study.The primary objective of this work was to investigate male forms and sunflower hybrids of the first generation for the contents of different tocopherol isomers. Segregation of the first generation hybrids was also investigated, and lines with increased contents of β, γ and δ-isomers of tocopherols were selected.

Material and methods.The studies were carried out in lines with high contents tocopherol isomers and in lines developed in the Laboratory of Sunflower Breeding and Genetics of the Plant Production Institute named after VYaYuriev of NAAS.

The tocopherol content and composition were analyzed by high-performance liquid chromatography.

Results and discussion.The article presents the results of studying the contents of tocopherol isomers in sunflower lines bred at the PPI as well as in lines developed in collaboration with the All-Russian Research Institute of Oil Crops. It was established that α-tocopherol predominated in sunflower lines bred at the PPI. Its content varied from 4.12 mg% (Kh279V) to 12.36 mg% (Kh220V). Lines developed in collaboration with the All-Russian Research Institute of Oil Crops containd a contrasting pattern of tocopherol isomers, which allows using them in sunflower breeding to obtain accessions with various contents of tocopherol isomers. The content of α-tocopherol ranges from 3.73 mg% (Vk-L-2) to 10.14 mg% (Vk-L-5); of β-tocopherol - from 1.08 mg% (Vk-L-2) to 22,52 mg% (Vk-L-4); of γ-tocopherol - from 0.54 mg% (Vk-L 1) to 6.60 mg% (Vk-L-4); and of δ-tocopherol - from 0.53 mg % (Vk-L-4) to 21.80 mg% (Vk-L-1).

Peculiarities of the inheritance of different isomers of tocopherols in the first generation hybrids were also studied. Tests showed that the content of α-tocopherol inherited by overdominance in all hybrid combinations.

Conclusions.It was found that various contents of tocopherols in the first-generation hybrids depended on male components. Choosing male components, one can obtain the first generation hybrids with different composition of tocopherol isomers